Figure 1.K6001-B7 carries a monogenic and dominant allele that confers resistance to cumene hydroperoxide (CHP).(A) K6001-B7 is
resistant to CHP. Overnight cultures of the parent (K6001, MATa),
K6001-B7 and an isogenic MATα
(K6001-α32) strain were
spotted as serial dilution on SC agar with and without CHP and grown at
28°C (upper panel). K6001-B7 grows on 0.1 mM CHP, a concentration lethal
for K6001 and K6001-α32. (lower
panel) Similar test were performed with oxidants tert-butyl hydroperoxide
(t-BTH) (left) and H2O2 (right). (B) K6001-B7
transmits with a monogenic Mendelian trait. K6001-B7 was mated with
K6001-α32, tetrads
gained by sporulation and tested for CHP and t-BTH resistance. Shown is the
2:2 segregation of a representative tetrade. (C) B7 is a dominant
mutation. (upper panel) K6001 and K6001-B7 were mated with
K6001-α32, resultant
diploids assayed for CHP resistance. +/B7 diploids retained the stress
resistance of the B7 haploid. (lower panel) B7 is dominant across
backgrounds. K6001 and K6001-B7 were mated with the distant S.
cerevisiae strain BY4742. Similar to the pure K6001 background
situation, BY/K6001-B7 diploids were more resistant to CHP (left) and
sensitive to H2O2 (right) compared to the BY/K6001
diploids.
