Figure 1. (A)
Survival assay displaying progressive loss viability of nutrient-repleted Phoenix
cells in serum free medium, and protection by either glucose or aminoacid
deprivation. Values are Mean±SD
of triplicate samples from one of several independent experiments. (B)
Effect of metabolic inhibitors on cell death by serum deprivation in
nutrient-rich medium. Death in the presence of serum was marginal, not
affected by inhibitors and is therefore displayed only for the 2 g/l
glucose sample. Extent of cell death in the absence of glucose is also
reported. Values are Mean±SD
of triplicate samples. Panel representative of several independent
experiments with very similar results. (C) Growth curves for Phoenix
cells grown in the absence of serum with or without nutrients. Numbers
refer to live cells, based on morphological features and trypan blue
exclusion. Values are Mean±SD
of triplicate samples. Panel representative of two independent experiments.
(D) Determination of ATP content in cells incubated for 24 hours in
the indicated conditions. Values are % of the control (1 g/l glucose +
aminoacids) sample. Chemiluminescence values were normalized for protein
content of the different samples. Representative of two independent
experiments.
