Research Paper Volume 2, Issue 8 pp 487—503

Figure 1. (A) Survival assay displaying progressive loss viability of nutrient-repleted Phoenix cells in serum free medium, and protection by either glucose or aminoacid deprivation. Values are Mean±SD of triplicate samples from one of several independent experiments. (B) Effect of metabolic inhibitors on cell death by serum deprivation in nutrient-rich medium. Death in the presence of serum was marginal, not affected by inhibitors and is therefore displayed only for the 2 g/l glucose sample. Extent of cell death in the absence of glucose is also reported. Values are Mean±SD of triplicate samples. Panel representative of several independent experiments with very similar results. (C) Growth curves for Phoenix cells grown in the absence of serum with or without nutrients. Numbers refer to live cells, based on morphological features and trypan blue exclusion. Values are Mean±SD of triplicate samples. Panel representative of two independent experiments. (D) Determination of ATP content in cells incubated for 24 hours in the indicated conditions. Values are % of the control (1 g/l glucose + aminoacids) sample. Chemiluminescence values were normalized for protein content of the different samples. Representative of two independent experiments.