Figure 1.Starvation impairs sleep initiation but not maintenance. (A,B)
A Drosophila activity monitor typically used for sleep studies
can record up to 32 flies simultaneously. An individual fly is housed in
each vertical tube and an infrared beam detects activity. The large
horizontal tubes contain either food (yellow) or agar (translucent).
Sliding barriers control access to each substrate [32]. Both tubes
contain food for fed controls (A, top), while agar is provided to
the starved experimental group (A, bottom; and B) on day 2 of testing the
experiment (starved, experimental). (C,D) Female flies
starved for 24 hours sleep less than fed counterparts. Shaded area (C)
represents lights-off. (E, F) The total number of sleep
bouts (Bout #) is decreased in starved flies while average bout length does
not differ from fed counterparts. Asterisk denotes significant difference
(P<0.01, ANOVA) from control groups. Data are mean ± SEM.
