SIRT1 reduces endothelial activation without affecting vascular
                        function in ApoE-/- mice

Sokrates Stein; Nicola Schäfer; Alexander Breitenstein; Christian Besler; Stephan Winnik; Christine Lohmann; Kathrin Heinrich; Chad E. Brokopp; Christoph Handschin; Ulf Landmesser; Felix C. Tanner; Thomas F. Lüscher; Christian M. Matter

doi:doi:10.18632/aging.100162

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Research Paper Volume 2, Issue 6 pp 353—360

SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice

Figure 1. (A) No difference in relaxation of aortic rings preconstricted with norepinephrine to the vasodilator acetylcholine. % Relaxation = % of precontraction to norepinephrine. (B) Relaxation of aortic rings at increasing sodium nitroprusside concentrations after norepinephrine precontraction. % Relaxation = % of precontraction to norepinephrine. (C) Contraction of aortic rings at increasing norepinephrine concentrations. % Contraction = % of contraction to 80 mM KCl. ApoE^-/- SIRT1^+/- (blue line) and ApoE^-/- SIRT1^+/+ (black line). (D) Aortic protein levels of total eNOS and phospho-eNOS (Ser1177). ApoE^-/- SIRT1^+/- (+/- and black columns) and ApoE^-/- SIRT1^+/+ (+/+ and white columns). n=6 per genotype