Research Paper Volume 2, Issue 6 pp 344—352
The choice between p53-induced senescence and quiescence is determined in part by the mTOR pathway
Figure 4. EEffect of rapamycin on nutlin-induced senescence in melanoma cells. (A) Mel-10 cells were incubated with 2.5 and 10 uM nutlin with or without 500 nM rapamycin for 1 day and then immunoblot was performed. (B) Beta-Gal staining. Mel-10 cells were incubated with 10 M nutlin alone and 500 nM rapamycin for 4 days, then drugs were washed out and cells were incubated for additional 3 days and stained for beta-Gal. Bars 50 um.