Figure 2.Depletion of TSC2 converts quiescence into senescence in WI-38tert cells. (A)Immunoblot.
WI-38tert cells were transduced with shTSC or control pLKO and cultured for
5 days. (B) WI-38tert
cells were transduced with lentiviruses. Next day, medium was replaced and
Nutlin (10 uM) with our without rapamycin was
added. After 4 days cells were washed and stained for beta-Gal. Bars 50 um.
(C) WI-38tert
cells were transduced with lentiviruses. Next day, medium was replaced and
Nutlin (10 uM) was added. After 4 days cells were washed and counted after
6 days.
