Figure 2.Effects of AOX expression on resistance to respiratory chain inhibitors. (A) Survival
after exposure to 3
mM rotenone or 3 mM antimycin A, of flies of strains and sexes indicated
(AOX / -, flies transgenic for UAS-AOX in absence of GAL4 driver; AOX /
da-GAL4, flies transgenic for AOX in presence of da-GAL4 driver). (B)
Representative oxygraph traces of mitochondrial suspensions (0.5 mg/ml in
state 3) in presence of inhibitors shown. Inferred oxygen consumption rates
(nmol/min) as indicated. Pyruvate+proline was used as substrate in all
experiments. (C) mtROS production (mean +
SEM) in presence of
inhibitors (at least 4 independent samples per experiment, a, b denote
significantly different groups, ANOVA, p < 0.05).
