Research Paper Volume 2, Issue 4 pp 200—223

Figure 2. Effects of AOX expression on resistance to respiratory chain inhibitors. (A) Survival after exposure to 3 mM rotenone or 3 mM antimycin A, of flies of strains and sexes indicated (AOX / -, flies transgenic for UAS-AOX in absence of GAL4 driver; AOX / da-GAL4, flies transgenic for AOX in presence of da-GAL4 driver). (B) Representative oxygraph traces of mitochondrial suspensions (0.5 mg/ml in state 3) in presence of inhibitors shown. Inferred oxygen consumption rates (nmol/min) as indicated. Pyruvate+proline was used as substrate in all experiments. (C) mtROS production (mean + SEM) in presence of inhibitors (at least 4 independent samples per experiment, a, b denote significantly different groups, ANOVA, p < 0.05).