Figure 2.MRC-5 normal human diploid fibroblasts were CYB5R3-silenced (KO cells) and cultured in DMEM medium supplemented with FBS 10%. (A) Cell growth and CYB5R3 protein
levels after five days of CYB5R3-silencing are shown. (B)
Oxygen consumption was measured in parallel in both control and CYB5R3-KO
cells. (C) Percentage of senescence was determined by
senescence-associated-β-galactosidase activity. (D)
Total RNA was extracted in both control and CYB5R3-KO cells and PGC1α mRNA
levels were obtained by real time PCR.
