Research Paper Volume 2, Issue 1 pp 43—62

Figure 3. mIGF-1 prevents Ang II- and IGF-1-induced cell hypertrophy (MF-20 staining) in HL-1 cardiomyocytes. (A) HL-1 cardiomyocytes were transfected or treated as in Legend of Figure 2A. Sarcomeric myosin was stained with MF-20 antibody and images were acquired using a Leica confocal microscope. (B) Cell size and cell hypertrophy quantified according to MF-20 staining in HL-1 cardiomyocytes in the different experimental conditions as in as in Legend of Figure 2A. Results are means ± SE of 3 independent experiments (^*,**p versus unstimulated control cells). Bar: 25 μM.