Priority Research Paper Volume 1, Issue 12 pp 971—978
Aged mouse ovaries possess rare premeiotic germ cells that can generate oocytes following transplantation into a young host environment
Figure 4. Induction of ovarian Stra8 expression in adult female mice is correlated with oocyte renewal. (A) Number of non-atretic immature follicles in ovaries of 2-month-old mice at the indicated times following a single intraperitoneal injection of DXR (mean ± SEM, n = 4 mice per group). (B) RT-PCR analysis of Stra8 expression in contralateral ovaries of 2-month-old mice at the indicated times following DXR injection (β-actin, house-keeping gene used as a sample loading control). (C, D) Examples of STRA8-immunopositive cells (green) in the surface epithelial layer of ovaries of mice 30 hours after DXR injection. Scale bar = 5 μm.