Priority Research Paper Volume 1, Issue 12 pp 971—978
Aged mouse ovaries possess rare premeiotic germ cells that can generate oocytes following transplantation into a young host environment
Figure 2. Young adult female mice support oocyte formation from germ cells in aged mouse ovaries. (A-F) Dual immunofluorescence analysis of GFP (green) and NOBOX (red, nucleus) expression in ovaries of young adult wild-type female mice (A, B), young adult TgOG2 female mice (C, D), and young adult wild-type recipient mice 6 weeks after proximal intrabursal grafting of aged TgOG2 ovarian tissue (E, F) (scale bar = 10 μm; DAPI, blue, nucleus). (G) Immunohistochemical detection of GFP (brown; upper), or dual immunofluorescence analysis of GFP (green) and NOBOX (red, nucleus) expression (middle; DAPI, blue, nucleus), along with numbers of non-follicle-enclosed GFP-positive germ cells and follicle-enclosed GFP-positive oocytes, in ovaries of wild-type young adult recipients treated with vehicle or TSA (recipient mouse #1-#3 or #4-#6, respectively) after proximal intrabursal grafting of aged TgOG2 ovarian tissue (scale bar = 10 μm).