Figure 2.Young adult female mice support oocyte formation from germ cells in aged mouse ovaries.
(A-F) Dual immunofluorescence analysis of GFP (green) and
NOBOX (red, nucleus) expression in ovaries of young adult wild-type
female mice (A, B), young adult TgOG2 female mice (C, D), and
young adult wild-type recipient mice 6 weeks after proximal intrabursal
grafting of aged TgOG2 ovarian tissue (E, F) (scale bar = 10 μm; DAPI, blue, nucleus). (G)
Immunohistochemical detection of GFP (brown; upper), or dual
immunofluorescence analysis of GFP (green) and NOBOX (red, nucleus)
expression (middle; DAPI, blue, nucleus), along with numbers of
non-follicle-enclosed GFP-positive germ cells and follicle-enclosed
GFP-positive oocytes, in ovaries of wild-type young adult recipients
treated with vehicle or TSA (recipient mouse #1-#3 or #4-#6, respectively)
after proximal intrabursal grafting of aged TgOG2 ovarian tissue (scale bar
= 10 μm).
