Figure 5.Chronic stimulation-induced adaptations in mitochondrial function and anti-apoptotic cell death signaling in young and old animals. (A).and (B) State 4 (2 μM rotenone and 10 mM succinate
as substrates) and state 3 (rotenone and succinate plus 0.44 mM ADP) rates
of oxygen consumption (VO2) in isolated subsarcolemmal (SS; A)
and intermyofibrillar (IMF; B) mitochondria from the control (CON,
open bars) and chronically stimulated (STIM, closed bars) limbs of young
and old animals (n = 6-10). (C) and (D) State 4 and
state 3 rates of reactive oxygen species (ROS) production per natom oxygen
consumed in SS (C) and IMF (D) mitochondria from the CON and
STIM limbs of young and old animals (n = 7-10). (E) Level of
fragmented DNA, in the form of mono- and oligonucleosomes, in
myonuclei-containing cytosolic extracts isolated from young and old animals
(n: STIM = 4, CON = 21). Data represent the mean ± SEM. * P <
0.05 vs. Young; ¶ P < 0.05 vs. CON).
