Research Paper Volume 1, Issue 9 pp 784—802
Dermal fibroblasts from long-lived Ames dwarf mice maintain their in vivo resistance to mitochondrial generated reactive oxygen species (ROS)
Figure 7. The antioxidant, N-acetyl cysteine (NAC) attenuates the ROS-mediated activation of p38 MAPK by ROT in young and old wild-type and Ames dwarf fibroblasts. The bar graphs and Western blot analyses below each bar graph show a time course of the effects of ROT (20 μM and NAC + ROT treatment on the induction of phosphorylation of the p38 MAPK catalytic site residues, P-Thr180/P-Tyr182, in young (4-6 mos) wild-type and Ames dwarf fibroblasts, and (B) in old (21-24 mos) wild type and Ames dwarf fibroblasts. n=4; * p<0.05 between wild-type and dwarf.