Figure 6.Fibroblasts from young (4-6 mos) and old (21-24 mos) dwarf mice show resistance to rotenone-induced p38 MAPK activation. Induction of
phosphorylation of the p38 MAPK catalytic site amino acid residues (P-Thr180/P-Tyr182)
by ROT is significantly lower in fibroblasts derived from young and old
dwarf mice. (A) The time course of activation of the p38 MAPK
catalytic site by rotenone (5 μM
and 20 μM) in
fibroblasts from young (4-6 mos) wild-type and Ames dwarf mice shows the
differences in levels of response to 5 μM
and 20 μM ROT. (B)
A time course of the activation of old (21-24 mos) wild-type and dwarf
mouse fibroblasts in response to 5 μM
and 20 μM ROT shows that
the level of activation of p38 MAPK catalytic site in aged wild-type and
dwarf fibroblasts increases with age. The Western blot time course
analyses below each bar graph show that the phosphorylated levels of the
p38 MAPK catalytic site are significantly lower in Ames dwarf fibroblasts
than their sage matched wild-type controls. n = 4; * p<0.05
between wild-type and dwarf.
