Figure 11.The effects of H 2O2 on the phosphorylation of p38 MAPK catalytic site in wild-type and Ames dwarf mouse fibroblasts. The fibroblasts from young (4-6mos) and old (21-24
mos) wild type and dwarf mice show an attenuated induction of
phosphorylation of the p38 MAPK catalytic site amino acid residues in
response to H2O2. The bar graphs and western blot
analyses below each bar graph show the pool levels and levels of
phosphorylation of the p38 MAPK catalytic site amino acid residues (P-Thr180/P-Tyr182)
in (A) young wild-type and dwarf fibroblasts (4-6 mos) and (B)
in old wild-type and dwarf fibroblasts (21-24 mos) after treatment with
varying concentrations of H2O2 (0.2 to 1.0 mM). The
p38 MAPK activities of both young and old wild type fibroblasts continue to
rise exponentially up to the highest level of H2O2 (1
mM) treatment. The fibroblasts from young and old dwarf mice show similar
levels of resistance to increasing levels of H2O2
suggesting that resistance to this ROS species does not change with age in
these fibroblasts. n=3, * p<0.05 between wild-type vs. dwarf.
