Research Paper Volume 1, Issue 9 pp 784—802

Figure 10. The young and old Ames dwarf fibroblasts show an attenuated phosphorylation of p38 MAPK catalytic site in response to antimycin A. The bar graphs and western blot analyses below each bar graph show the pool levels and the levels of phosphory-lation of the p38 MAPK catalytic site amino acid residues (P-Thr¹⁸⁰/P-Tyr¹⁸²) in (A) young wild-type and dwarf fibroblasts (4-6 mos) and (B) old wild-type and dwarf fibroblasts (21-24 mos) after treatment with 50 μM AA. The fibroblasts from young dwarf mice show an attenuated response to the AA-mediated phosphorylation of the catalytic site. The fibroblasts from young and old dwarf mice show similar levels of resistance to AA suggesting that resistance to CIII generated ROS does not change with age in these fibroblasts. n=3, *p<0.05 between wild-type vs. dwarf.