Figure 1.Loss of SIRT1 has minimal impact on gluconeogenesis in primary hepatocytes. (A) Glucose output
from primary hepatocytes isolated from control and SIRT1 LKO mice. Cells
were treated with DMSO (white bars) or 10 μM forskolin (black bars) and
incubated for 6 h in glucose free DMEM supplemented with 20 mM sodium
lactate and 2 mM sodium pyruvate. Glucose output was measured in culture
medium using a glucose oxidase kit (Sigma). Data represent mean +
SD. (B-C) SIRT1 deficiency in primary hepatocytes reduces the
induction of PGC-1α (B) but not
PEPCK (C) message in response to 10μM forskolin treatment. mRNA from
primary hepatocytes treated with DMSO (white bars) or forskolin (black
bars) were analyzed using qPCR. Data represent mean +
SD.
