Research Paper Volume 1, Issue 9 pp 771—783
Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle
Figure 1. Tissue distribution of SIRT3 protein. The SIRT3 protein is abundantly expressed in the brown adipose tissue (BAT), liver, kidney, heart, brain, and soleus muscle, but very low in white adipose tissue (WAT), the extensor digitorum longus muscle (EDL), or the gastrocnemius muscle (Gastro). For each sample, 50 μg of protein was loaded into a 10% acrylamide gel, electrophoresed, and transferred to a nitrocellulose membrane. The membrane was probed using an anti-SIRT3 serum or an anti-β-actin antibody. Blots were quantified with ImageQuant and SIRT3/actin ratios are provided; since gastrocnemius (Gastro) has the lowest SIRT3 expression in vivo, normalization (l.0) was set with respect to this tissue.