Figure 1.Constitutive UCP expression in adult IPCs results in decreased steady state intracellular Ca 2+ levels. (A) Under normal growth conditions, the Cg-2 protein in the
adult IPCs produces fluorescent signals reflecting a steady state Ca2+
flux whereas a 21-51% decrease in average fluorescent intensity is detected
in adult IPCs of two dilp2-Gal4/UAS-mucp1, UAS-cg-2 co-expressing
fly lines (mUCP1-1 and mUCP1-2), consistent with attenuated insulin
action. Each bar represents mean +
S.E.M. *p=0.0004, **p<0.0001
(Student's t-test). Shown are
average fluorescent signals from IPCs of 10 brains of each line in a
representative experiment (see Materials and Methods). Reproducible
results were obtained in three independent experiments. Representative
images of IPCs from a control adult brain isolated from a dilp2-Gal4/UAS-cg-2 fly line (B)
and an adult brain isolated from a dilp2-Gal4/UAS-mucp1, UAS-cg-2 fly line (C)
are shown. Images were taken with a 40X objective. Scale bar, 100 μm.
