Figure 5. Double mutant
experiments of afo1Δ
and
tor1Δ. The TOR1 gene is involved
in nutrient sensing and lifespan determination in yeast [5]. The double
mutant was constructed in an isogenic cross between the two single mutants
in the BY background. Lifespans of the wild type, both single mutants and
the double mutant were determined by micromanipulation. The experiment
shows that an intact TOR1 gene is needed for the lifespan
elongation observed in the afo1Δ strain as the
lifespan of the afo1Δ,
tor1Δ double
mutant strain is not significantly different (p<0.02) from the lifespan
of the tor1Δ
single
mutant strain.
