Research Paper Volume 1, Issue 7 pp 622—636

Figure 5. Double mutant experiments of afo1Δ and tor1Δ. The TOR1 gene is involved in nutrient sensing and lifespan determination in yeast [5]. The double mutant was constructed in an isogenic cross between the two single mutants in the BY background. Lifespans of the wild type, both single mutants and the double mutant were determined by micromanipulation. The experiment shows that an intact TOR1 gene is needed for the lifespan elongation observed in the afo1Δ strain as the lifespan of the afo1Δ, tor1Δ double mutant strain is not significantly different (p<0.02) from the lifespan of the tor1Δ single mutant strain.