Research Paper Volume 1, Issue 7 pp 622—636

Figure 4A. Influence of rapamycin on subcellular localization of the transcription factor, Sfp1p. Strains were grown in liquid SC+2% glucose at 28°C until early logarithmic phase and rapamycin was added to a final concentration of 100 nM. This concentration is growth inhibitory for the wild type strain [6]. Confocal images were taken at time zero (before addition of rapamycin) and at 4 h. The chromosomally integrated SFP1-GFP-HIS3 construct [37] was present in the wild type strain BY4741, was PCR cloned, sequenced and chromosomally integrated at the SFP1 locus in strains afo1Δ and BY4741 ρ°, respectively.