Figure 4A.Influence of rapamycin on subcellular
localization of the transcription factor, Sfp1p. Strains
were grown in liquid SC+2% glucose at 28°C until early
logarithmic phase and rapamycin was added to a final
concentration of 100 nM. This concentration is growth
inhibitory for the wild type strain [6]. Confocal images
were taken at time zero (before addition of rapamycin)
and at 4 h. The chromosomally integrated SFP1-GFP-HIS3
construct [37] was present in the wild type strain BY4741,
was PCR cloned, sequenced and chromosomally integrated at
the SFP1 locus in strains afo1Δ and BY4741 ρ°, respectively.
Figure 4A — The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1 | Aging
