Figure 3.Resveratrol rescues low activity of the flies on high-calorie diet. (A)
Effect of 50 μM,
100 μM and 200 μM of resveratrol
in 0.5X (0.5X 50Res, 0.5X 100Res, 0.5 200Res) and 1.5X (1.5 50Res, 1.5
100Res, 1.5X 200Res) food on CS male spontaneous physical activity
compared to activity of flies on 0.5X and 1.5X food that contain ethanol
(0.5 EtOH and 1.5 EtOH) used as resveratrol solvent. (B) Total
daily spontaneous physical activity of male CS flies on 0.5X with
200 μM resveratrol
(0.5 200Res) and 1.5X with μM
200 resveratrol (1.5 200Res) compared to the male flies on 0.5X and 1.5X
food that contain ethanol (0.5 EtOH and 1.5 EtOH). The data are mean total
24s spontaneous activities collected independently for 3 vials with 10
flies each collected at age 9 (A) and 6 (B) days, except in A
where there were 6 vials of 0.5X EtOH and 1.5X EtOH. A Tukey HSD post-hoc
test was conducted on the food means to determine which means are pairwise
statistical different from one another. Results of statistical analysis are
in Supplementary Table 1A and Supplementary Table 1B. (C) Male yw wild type flies on 1.5X
food with addition of 200 μM
of resveratrol (1.5 200Res) have the highest activity compared to the flies
on 1.5X EtOH, 0.5X 200Res and 0.5X EtOH. Flies were 3 (0.5X 200Res and 0.5X
EtOH) and 4 (1.5XRes and 1.5EtOH) days old. Statistical significance was
determined by using two-tailed Student's t-test with unequal variances.
