Figure 4.Resveratrol inhibits S6K1 in vitro.(A) Structural
similarity between resveratrol and quercetin, a known kinase inhibitor. (B) Kinase
inhibition profile for resveratrol at 20 μM obtained using
KinaseProfiler™ (Upstate). Dashed line represents 100% activity as compared
to control. Black filled-in bar on the graph indicates S6K1. Complete data
set is provided in Supplementary Table 1. Error bars represent s.d.m. (C) Phosphorylation
of recombinant GST-tagged S6 by immunoprecipitated HA-S6K1 under increasing
concentrations of resveratrol (Res). Autoradiograph depicts S6K1
phosphorylation of GST-S6. (D) Average of three separate kinase assay
experiments as performed in (C). Densitometry was performed using
NIH ImageJ. Error bars represent s.e.m.
