Research Paper Volume 1, Issue 5 pp 490—502
The regulation of p53 by phosphorylation: a model for how distinct signals integrate into the p53 pathway
Figure 5. Activation of p53 by viral infection: effects of a CK1 inhibitor on p53 phosphorylation. (A) A CK1 inhibitor attenuates Ser20 site phosphorylation of p53 and p53 induction mediated by HHV-6B infection. MOLT-3 cells were infected with (even-numbered lanes) or without (odd-numbered lanes) HHV-6B for 48 hours in the presence of increasing concentrations (10-100μM) of the CK1 inhibitor D4476 (lanes 3-14) or a DMSO solvent control (lanes 1-2). Cell lysates were examined by Western blotting with antibodies against the indicated proteins. (B) A CK1 inhibitor does not attenuate Ser20 site phosphorylation of p53 nor p53 induction mediated by treatment with X-rays. MOLT-3 cells were treated with (even-numbered lanes) or without (odd-numbered lanes) 6Gy X-ray and cultured for 4 hours after an initial 44-hour pre-treatment with: increasing concentrations (10-60μM) of the CK1 inhibitor D4476 (lanes 5-12), a DMSO solvent control (lanes 3-4), or a culture medium control (lanes 1-2). Cell lysates were examined by Western blotting with antibodies against the indicated proteins.