Figure 5.Activation of p53 by viral infection: effects of a CK1 inhibitor on p53 phosphorylation.(A) A CK1 inhibitor attenuates
Ser20 site phosphorylation of p53 and p53 induction mediated by HHV-6B
infection. MOLT-3 cells were infected with (even-numbered lanes) or without
(odd-numbered lanes) HHV-6B for 48 hours in the presence of increasing
concentrations (10-100μM) of the CK1 inhibitor D4476 (lanes 3-14) or a
DMSO solvent control (lanes 1-2). Cell lysates were examined by Western
blotting with antibodies against the indicated proteins. (B) A CK1 inhibitor does not attenuate Ser20 site
phosphorylation of p53 nor p53 induction mediated by treatment with X-rays.
MOLT-3 cells were treated with (even-numbered lanes) or without
(odd-numbered lanes) 6Gy X-ray and cultured for 4 hours after an
initial 44-hour pre-treatment with: increasing concentrations
(10-60μM) of the CK1 inhibitor D4476 (lanes 5-12), a DMSO solvent
control (lanes 3-4), or a culture medium control (lanes 1-2). Cell lysates
were examined by Western blotting with antibodies against the indicated
proteins.
