Research Paper Volume 1, Issue 5 pp 470—480

Figure 1. Activities of the p21 promoter-luciferase constructs with the common (p21C-luc; black bars) or rare (p21R-luc; grey bars) allele SNP cluster in the -4547/-3489 bp region. (A) Three independent plasmid preparations of each of p21C-luc or p21R-Luc (R) were transfected into HCT116 wild type (WT) cells. Cells were harvested 48 h after transfection, and firefly luciferase activity was measured and normalized to Renilla luciferase expressed from a co-transfected construct. The bars show mean and standard deviation for triplicate transfections. (B) p21C-luc and p21R-luc plasmids were transfected in parallel into HCT116 WT and p53-/- cell lines, in triplicates as in A. The bars show mean and standard deviation of the ratio of normalized luciferase activities achieved with the same plasmid in the WT relative to p53-/- cells.