Figure 1. Activities of
the p21 promoter-luciferase constructs with the common (p21C-luc; black
bars) or rare (p21R-luc; grey bars) allele SNP cluster in the -4547/-3489
bp region. (A) Three independent plasmid preparations of each of p21C-luc or
p21R-Luc (R) were transfected into HCT116 wild type (WT) cells. Cells were
harvested 48 h after transfection, and firefly luciferase activity was
measured and normalized to Renilla luciferase expressed from a
co-transfected construct. The bars show mean and standard deviation for
triplicate transfections. (B) p21C-luc and p21R-luc plasmids were
transfected in parallel into HCT116 WT and p53-/- cell lines, in
triplicates as in A. The bars show mean and standard deviation of the ratio
of normalized luciferase activities achieved with the same plasmid in the
WT relative to p53-/- cells.
