Research Paper Volume 1, Issue 4 pp 425—437

Figure 2. Alterations in the energetics of Atg7 ^-/- MEFs. (A) Western blot analysis of wild type (+/+) or Atg7^-/- MEFs for the expression of Atg7, p62 and actin (loading control). (B) Measurement of oxygen consumption for WT and Atg7^-/- MEFs under basal conditions, following the addition of the mitochondrial electron chain inhibitor oligomycin (0.5 μM), or in the presence of the mitochondrial uncoupler FCCP (1 μM), to determine maximal oxidative capacity. Shown is the average fold change +/- SEM in oxygen consumption (WT MEFs basal respiration=1) obtained from 5 experiments each performed in triplicate. (C) Assessment of mitochondrial number in WT or Atg7^-/- MEFs. DNA was isolated from WT (n=3 independent WT MEF cell isolates) and Atg7^-/- MEFs (n=3 independent Atg7^-/- MEF cell isolates) and quantitative PCR analysis performed for the mitochondrial-encoded gene ND1 and the nuclear-encoded gene H19. (D) Relative extracellular acidification rates indicating lactic acid production and hence glyolytic rates in WT or Atg7^-/- MEFs. Shown is the average +/- SEM fold change in lactic acid production from 8 experiments each performed in triplicate. * p≤0.05; ** p≤0.01.