Figure 6.Effect of Resveratrol and DHEA on phosphorylation of AMPK. Panel A,
SaOS2 cells were treated with increasing concentrations of resveratrol for
48 hours, after what, proteins were extracted and probed either with
anti-phospho-AMPK (Thr172), or with antibody to AMPK. Panel B, RGC
cells were incubated with 20 or 100 μM
resveratrol or DHEA 100 μM
for 48 hours, and then probed for phosphor-AMPK and AMPK as described
above.