Research Paper Volume 1, Issue 4 pp 412—424
Dual targeting of the antagonistic pathways mediated by Sirt1 and TXNIP as a putative approach to enhance the efficacy of anti-aging interventions
Figure 6. Effect of Resveratrol and DHEA on phosphorylation of AMPK. Panel A, SaOS2 cells were treated with increasing concentrations of resveratrol for 48 hours, after what, proteins were extracted and probed either with anti-phospho-AMPK (Thr172), or with antibody to AMPK. Panel B, RGC cells were incubated with 20 or 100 μM resveratrol or DHEA 100 μM for 48 hours, and then probed for phosphor-AMPK and AMPK as described above.