Research Paper Volume 16, Issue 7 pp 6566—6587

Transcranial direct current stimulation promotes angiogenesis and improves neurological function via the OXA-TF-AKT/ERK signaling pathway in traumatic brain injury

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Figure 6. tDCS enhanced angiogenesis after TBI through OXA-TF-AKT/ERK pathway. (A) Representative western blotting images of TF, p-TF, AKT, p-AKT, ERK and p-ERK on 14 days post-TBI. Using GAPDH and β-Tubulin as internal reference for band density normalization (n = 3). (B) Quantification of western blotting for p-TF/TF expression (n = 3). (C) Quantification of western blotting for p-AKT/AKT expression (n = 3). (D) Quantification of western blotting for p-ERK/ERK expression (n = 3). Results are expressed as means ± standard deviation, the statistical significance of differences was evaluated by One-way ANOVA, *denotes the comparison between the Sham group and the TBI group, #represents the comparison between the TBI and the Sham-tDCS group, &signifies the comparison between the tDCS group and the Sham-tDCS group, and ¥indicates the comparison between the tDCS group and the tDCS+SB334867 group. P < 0.05 indicates statistical significance.