Research Paper Volume 16, Issue 7 pp 6566—6587

Figure 2. tDCS promotes the expression of OXA and OX1R in TBI rats. (A) Representative western blotting images of Orexin-A and Orexin-A receptor 1 (OX1R) on 14 days post-TBI. Using GAPDH as an internal reference for band density normalization (n = 3). (B) Quantification of western blotting for Orexin-A expression (n = 3). (C) Quantification of western blotting for OX1R expression (n = 3). (D) Representative immunofluorescence images of OX1R expression in brain tissue (n = 3). (E) Quantification of immunofluorescence for OX1R expression (n = 3). (F) Quantitative analysis of mRNA levels of OX1R expression (n = 3). Results are expressed as means ± standard deviation, the statistical significance of differences was evaluated by One-way ANOVA, ^*represents the comparison between the Sham group and the TBI group, ^#signifies the comparison between the TBI group and the tDCS group, and ^&denotes the comparison between the tDCS group and the Sham-tDCS group. P < 0.05 indicates statistical significance.