Research Paper Volume 16, Issue 8 pp 6773—6795

Figure 6. HAPSTR1 inhibits ubiquitination of LRPPRC and recruits PSMD14 to interact with LRPPRC. (A) Immunofluorescence staining was used to determine the common cellular localization of HAPSTR1 and LRPPRC. Original magnification: 400x. (B) The gels in the co-immunoprecipitation (COIP) assay were stained with Coomassie Brilliant Blue. (C) A COIP assay was used to detect the interaction between HAPSTR1 and LRPPRC in SKOV3 and A2780 cells. (D) HAPSTR promoted protein stability of LRPPRC under cycloheximide treatment. (E) HAPSTR1 suppressed ubiquitination of LRPPRC. (F) COIP was carried out to detect interactions between LRPPRC and HAPSTR1 and PSMD14 simultaneously. (G) HAPSTR1 recruited more PSMD14 for binding to LRPPRC.