Research Paper Volume 16, Issue 3 pp 2908—2933

Figure 4. Transcriptomic clustering of NSCLC patients from GSE148071 dataset. (A) Marker-based cell type identification analysis allowed the prediction of four broad cell types across all profiled single cells. (B) Expression levels of cell type signatures overlaid on the t-SNE representation. EPCAM for tumor cells, CD3E for T cells, CD14 for macrophages and CD1C for cDC. (C) Gene expression heatmap of top-10 cell type-specific marker genes as measured by Wilcoxon rank-sum test. (D) ARGRS overlaid on the t-SNE representation. (E) Boxplot showing the levels of ARGRS between tumor and non-tumor cells. Horizontal lines in the boxplots represent the median, the lower and upper hinges correspond to the first and third quartiles, and the whiskers extend from the hinge up to 1.5 times the interquartile range from the hinge. (F) Expression levels of the five genes in the prognostic model overlaid on the t-SNE representation.