Research Paper Volume 16, Issue 3 pp 2141—2160

Moxibustion prevents tripterygium glycoside-induced oligoasthenoteratozoospermia in rats via reduced oxidative stress and modulation of the Nrf2/HO-1 signaling pathway

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Figure 3. Effects of moxibustion on rat sperm DNA integrity detected using flow cytometry. (A) Representative images of CON, OAT, DM, and ADM groups. Each dot represents a spermatozoon characterized by the amount of double-stranded DNA (green fluorescence) or single-stranded DNA (red fluorescence). R2 = normal sperm population. R3 = DNA denatured sperm population. R4 = immature sperm population. (B) DNA fragmentation index (n = 6). ***P < 0.001.