Figure 3. Effects of moxibustion on rat sperm DNA integrity detected using flow cytometry. (A) Representative images of CON, OAT, DM, and ADM groups. Each dot represents a spermatozoon characterized by the amount of double-stranded DNA (green fluorescence) or single-stranded DNA (red fluorescence). R2 = normal sperm population. R3 = DNA denatured sperm population. R4 = immature sperm population. (B) DNA fragmentation index (n = 6). ***P < 0.001.