Research Paper Volume 16, Issue 1 pp 685—700
ZIP4 upregulation aggravates nucleus pulposus cell degradation by promoting inflammation and oxidative stress by mediating the HDAC4-FoxO3a axis
Figure 2. ZIP4 expression in the NP tissues of IDD patients. The NP tissues of IDD patients and normal patients were collected. (A) RT-PCR was used to ascertain the ZIP4 mRNA profile. (B) ZIP4 mRNA levels in different grades of IDD patients. (C) Correlation between ZIP4 mRNA level and grades of IDD patients. (D) Western blotting was used to detect ZIP4 protein level in the NP tissues of both IDD and normal patients. N=3. ***P<0.001.