Research Paper Volume 16, Issue 1 pp 617—626

GDF15 restrains myocardial ischemia-reperfusion injury through inhibiting GPX4 mediated ferroptosis

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Figure 4. pc-DNA-GDF15 significantly inhibited the ferroptosis in OGD/R-treated H9C2 cells. (A, B) The Fe2+ level in cells were validated (bar: 100 μm, magnification: 100×); (C, D) The relative expression intensity of GPX4 in the cells were evaluated (bar: 200 μm, magnification: 40×); (E, F) The levels of GSH and MDA in the cells were measured. *P < 0.05, **P < 0.01, ***P < 0.001.