Research Paper Volume 15, Issue 24 pp 15196—15212

Figure 5. Notch-1^MAC-KO reduced the secretion of PI3K and caused M2 anti-inflammatory effect. (A) Western blotting revealed that the expression levels of Notch-1, p-PI3K, p-AKT, CD36, SREBP-1 and Arg-1 were markedly enhanced in the peritoneal macrophages of Notch-1^MAC-KO mice, which were reversed by an anti-PI3K neutralizing antibody (LY-294002). (B) Quantitative analysis of relative protein levels revealed that Notch-1, p-PI3K, p-AKT and Arg-1 were increased in the peritoneal macrophages of K.O. group compared with W.T. group, which were significantly reversed by LY-294002. ^**P < 0.01, ns P > 0.05: peritoneal macrophages of Notch-1^MAC-KO mice vs. peritoneal macrophages of Notch-1^WT mice.