Research Paper Volume 15, Issue 23 pp 14172—14191

Figure 9. GP reverses dendritic spine loss and recovers postsynaptic proteins. (A, B) Representative Nissl staining images and the quantification of neuron number in the CA3 region of rat hippocampus, scale bar = 200 and 100 μm for low and high magnifications respectively. (C, D) Representative Golgi staining images and statistics analysis of spine number in the dentate gyrus region of rat hippocampus (scale bar, 10 μm). n = 5. (E–H) Western blots and quantitatively analysis for synaptic proteins (PSD95, GluR2 and synaptophysin) levels in rat hippocampus. Data represent mean ± SEM, p-value significance is calculated from one-way ANOVA, n = 3, ^*p < 0.05, ^**p < 0.01, ^***P < 0.001 and ^****P < 0.0001.