Research Paper Volume 15, Issue 23 pp 14019—14038

Figure 7. CHIP-overexpressing ADSCs (rADSC^CHIP-WT) in the aging-SHR model inhibit cardiac remodeling. (A) The immunoblotting detects the survival markers expression of heart tissue in different experimental groups after the transplantation. (B) Hematoxylin-eosin (HE) staining (upper panel) and Masson's trichrome staining (lower panel) were performed to evaluate tissue arrangement and collagen accumulation in different experimental groups. Scale bar = 100 μm. (C) Heart tissue mRNA expression levels of IGF1R, IGFBP3, and IGF1 in WKY, SHR, and SHR-treated groups were analyzed by qRT PCR analysis. (D, E) TUNEL assay to detect the apoptosis rate of different experimental groups. (N = 3; ^*p < 0.05; ^**p < 0.01 indicate significant differences).