Research Paper Volume 15, Issue 22 pp 13542—13557

Figure 5. MiR-641 can target PTEN in ¹³¹I-resistant TC cells. (A) The binding side prediction of PTEN and miR-641 in the ENCORI database. (B, C) The ¹³¹I -resistant TPC-1 and FTC-133 cells were treated with miR-641 mimic. (B) Luciferase reporter gene assays of PTEN mRNA 3’UTR luciferase activities. (C, D) The qPCR analysis of PTEN in the cells. (E) The Western blot analysis of miR-641 in ¹³¹I -resistant TPC-1 and FTC-133 cells treated with EGOT siRNA, or co-treated with EGOT siRNA and miR-641 inhibitor. mean ± SD, ** P < 0.01. Experiments were repeated at least biological triplicates.