Research Paper Volume 15, Issue 23 pp 13961—13979

Figure 4. Fibronectin, E-cadherin, and N-cadherin regulate cell adhesion, migration, and invasion. (A–C) NCI-H1975 and A549 cells were transfected with shRNAs targeting fibronectin and then analyzed by Western blotting, migration and invasion assay. (D–F) NCI-H1975 and A549 cells were transfected with shRNAs targeting E-cadherin and then analyzed by Western blotting, migration and invasion assay. (G–I) NCI-H1975 and A549 cells were transfected with shRNAs targeting N-cadherin and then analyzed by Western blotting, migration and invasion assay. The grouping of blots cropped from different parts of the different gels. Data are shown as the mean ± SD and repeated for three biologically independent experiments. P-values are shown; two-tailed Student’s t-test. Abbreviation: NC: negative control shRNA. Vector, vector-only control. The representative data are repeated for three biologically independent experiments. Student’s t-tests were used for data analysis; ^*P < 0.05, ^**P-value < 0.01, ^***P-value < 0.001 and ^****P-value < 0.0001.