Research Paper Volume 15, Issue 19 pp 10627—10639

Figure 6. Effect of different durations of ischemia on cardiac remodeling after reperfusion. (A) Representative images of HE and Masson’s trichrome staining and (B) quantification of fibrotic area (%) in mouse hearts treated as indicated (n = 4 : 4 : 4 : 4). Scale bar: 500 μm. (C) Representative western blot and (D–F) statistical data of myocardial fibrosis by detection of (D) α-SMA, (E) MMP-9, and (F) collagen I expression levels in mouse hearts treated as indicated (n = 3 : 3 : 3 : 3). MI=Myocardial infarction, Rep=reperfusion. Data are shown as mean ± SD. One-way ANOVA followed by Tukey post hoc test was used for statistical comparisons between multiple groups. *P < 0.05; **P < 0.01.