Research Paper Volume 15, Issue 19 pp 10428—10452
Mitochondrial dysfunction following repeated administration of alprazolam causes attenuation of hippocampus-dependent memory consolidation in mice
Figure 6. Effects of repeated administration of Alp on learning and memory in mice. (A) Scheme of behavioral experiments. (B) The drinking error of each mouse was examined by ICS (n = 12). (C) The learning ability of each mouse was evaluated by NOR (n = 12). (D) HE staining in each region (CA1, CA2, CA3 and DG) of the hippocampus. (E) Counting of pyramidal neurons in each region (CA1, CA2, CA3 and DG) of the hippocampus (n = 6). (F) Immunohistochemical staining in each region (CA1, CA2, CA3 and DG) of the BDNF expression. (G) Statistical results of BDNF immunohistochemical in average optical density (n = 6). Scale bar is 100 μm. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 vs. the control group; #p < 0.05, ##p < 0.01 vs. the Alp group.