Research Paper Volume 15, Issue 19 pp 10089—10104
Eriocitrin inhibits epithelial-mesenchymal transformation (EMT) in lung adenocarcinoma cells via triggering ferroptosis
Figure 3. Eriocitrin treatment triggered ferroptosis in A549 and H1299 cells via suppressing Nrf2 expression. (A) Fluorescent images of ROS in A549 and H1299 cells. (B–F) Protein expression of ferroptosis-related genes after 50 μM concentration eriocitrin treatment, including SLC7A11, GPX4, Nrf-2 and FTH1. (G) Relative iron levels of A549 and H1299 cells after 50 μM concentration eriocitrin treatment. (H, I) mRNA expression of ferroptosis-related genes after 50 μM concentration eriocitrin treatment, including GPX4 and FTH1. *P < 0.05, **P < 0.01, and ***P < 0.001.