Research Paper Volume 15, Issue 17 pp 9217—9229

Euphorbia factor L1 suppresses breast cancer liver metastasis via DDR1-mediated immune infiltration

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Figure 5. DDR1 overexpression blocked EFL1-induced beneficial effects in SHI mice. (A) Verification of DDR1 overexpression transfection using western blot analysis. n = 6. (B, C) Tumor-infiltrating CD4+, CD8+, CD49b+ T cells and Tregs were analyzed by flow cytometry. n = 3. (D, E) ALT and ALT activities in mouse serum. n = 6. (F, G) Abdominal circumference and peritoneal fluid volume of mice in each group. n = 9. Data were compared using unpaired Student’s t test (A) or two-way ANOVA with Tukey’s post hoc (BG). **P < 0.01. Abbreviation: ns: not significant.