Research Paper Volume 15, Issue 14 pp 6921—6932

Figure 6. Exosomal lnc-CDHR of hUMSCs competitively bound to miR-3149 and regulated the suppression of target PTEN genes to attenuate EMT in HMrSV5. (A) Transfection efficiency of miR-3149 mimic was detected by PCR. (B–E) A rescue experiment was implemented to figure out the relationship between the upstream and downstream of lnc-CDHR and miR-3149. (F) Bioinformatics analysis result showed that miR-3149 had a binding site with lnc-CDHR and PTEN. (G) Dual-luciferase reporter gene assay was used to analyze the relationship between lnc-CDHR and miR-3149. (H) Dual-luciferase reporter gene assay was used to analyze the relationship between miR-3149 and PTEN. Each value represents the mean ± SEM (n=3) (C–E) ^**P<0.01 vs. Control, (G) ^**P<0.01 vs. CDHR WT, (H) ^**P<0.01 vs. PTEN WT, ^##P<0.01 vs. HG, ^%%P<0.01 vs. HG+CDHR, ^%P<0.05 vs. HG+CDHR.